MANY previous investigations on antibody production in tissue culture have utilized indirect systems for antibody detection 1,2. Others have examined the incorporation of isotope-labelled amino-acids ...
A WEDGE-SHAPED agar gel is formed by pouring out 10 ml. of a hot 2 per cent agar solution (‘Bacto-Agar’, Difco Laboratories) on a pre-warmed glass plate (16 cm. × 4 cm.), which is slightly inclined.